解剖学报

›› 2010, Vol. 41 ›› Issue (6): 805-808.doi: 10.3969/j.issn.0529-1356.2010.06.005

• 论著 • 上一篇    下一篇

雷公藤内酯醇对阿尔茨海默病模型大鼠海马补体C1q表达的影响

杨宝林1，2;吕诚1*; 胡小令1;万斌1 ;蔡维君2 ;黄涛波1 ;桂婷1   

  1. 1.南昌大学医学院人体解剖学教研室，南昌 330006; 2.中南大学湘雅医学院人体解剖学与神经生物学系，长沙 410013
  • 收稿日期:2009-12-15 修回日期:2010-03-04 出版日期:2010-12-06
  • 通讯作者: 吕诚

Effect of triptolide on expression of C1q in the hippocampus of Alzheimer’s disease rats

  1. 1. Department of Anatomy, Medical School of Nanchang University, Nanchang 330006, China;2. Department of Anatomy and Neurobiology, Xiangya School of Medicine,Central South University, Changsha 410013, China
  • Received:2009-12-15 Revised:2010-03-04 Online:2010-12-06
  • Contact: LÜ Cheng

关键词: 雷公藤内酯醇, 阿尔茨海默病, C1q, 海马, 免疫组织化学, 反转录-聚合酶链式反应, 大鼠

Abstract: Objective To investigate the effect of triptolide(TP) on expression of C1q in the hippocampus of model rats with Alzheimer’s disease(AD). Methods Thirty male SD rats were randomly divided into control group, AD model group and TPtreated group.Each group had 10 rats. The AD model group was established with bilateral microinjection of aggregated beta-amyloid protein(Aβ)SUB>1-40/SUB> into hippocampus in rats and Morris water maze test was used to judge that the model was successful or not. The control group was established with bilateral microinjection of normal saline into hippocampus in rats.The TPreated group rats were administered TP ［0.4mg/(kg.d)］ intraperitoneally after the AD model was made successfully.The change of expression of C1q protein and mRNA were observed with immunohistochemical staining and RT-PCR 15 days later. Results Immunohistochemically, the cell number of C1q positive staining in hippocampus of the AD model group（67.89±9.22） increased obviously as compared with the control group（36.27±5.09）（EM>P/EM>0.01）, and their average optical density（0.2918±0.0347） increased obviously as compared with the control group（0.1980±0.0183）（EM>P/EM>0.01）, the average optical density of C1q positive

Key words: Triptolide, Alzhmeimer’s disease, C1q, Hippocampus, Immunohistochemistry, RT-PCR, Rat

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